Multilayer Graft Formed from the Decellularized Vessel and Bioresorbable Material as a Possible Vascular Prosthesis

Aleksandr V. Kriventsov ¹, Victor N. Aleksandrov ¹, Mariya A. Figurkina ², Sergey V. Kromskiy ², Ekaterina V. Mikhaylova ², Pavel V. Popryadukhin ³, Vasiliy S. Sidorin ², Gennadiy G. Khubulava ¹

¹ The S. M. Kirov Military Medical Academy, St. Petersburg, Russia, Saint-Petersburg, Russia; ² State Pediatric Medical University, St. Petersburg, Russia, Sankt-Peterburg, Russia; ³ Institute of Macromolecular Compounds, Russian Academy of Sciences, St. Petersburg, Russia, Sankt-Peterburg, Russia;

Date, time and location: 2018.05.26 08:30, Exhibition area, 1st Floor. Zone – B

Abstract

Objective:biocompatible and bioresorbable grafts have been investigated as potentialprostheses.However, prostheses from decellularized tissue (DCT) and poly L-lactide (PLLA) fail due to the formation of aneurysms. We aim to study the perspective of using multilayer graft, composed of outer DCT and inner BM layers, as a functional conduit.

Methods: Infrarenal aortas of Wistar rats (< 150 g) were replaced with grafts (n = 20). Ischemia time of the lower part of the body was about 40 min and anticoagulants were not used. After 6 months the diameter of the prosthesis and the mean linear velocity of blood flow were measured. Traditional histological and immunohistochemical methods were used for tissue staining.

Results: in the course of the experiment the lethality was not observed. The formation of an endothelial layer within the first month was shown using CD31 antibodies and scanning electron microscopy. The linear velocity of blood flow was reduced in comparison with intact animals in the absence of signs of constriction of the vessel. The signs of the tunica media formation in the BM layer were weakly expressed – rare disordered elastic fibers and presence of smooth muscle cells in both layers were shown.

Conclusions:our experiments provide evidence of potential clinical application of multilayer graft, which combines adequatemechanical properties with molecular composition of the extracellular matrix thus promoting cellular activity.

The work was supported by RNF (PLLA formation, 14-33-00003).